How does BODIPY work?

How does BODIPY work?

BODIPY and many of its derivatives have received attention recently for being fluorescent dyes with unique properties. They strongly absorb UV-radiation and re-emit it in very narrow frequency spreads, with high quantum yields, mostly at wavelengths below 600 nm.

What does BODIPY stand for?

The common name is an abbreviation for “boron-dipyrromethene”. It is a red crystalline solid, stable at ambient temperature, soluble in methanol.

What structure does BODIPY stain?

BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.

What does BODIPY FL stain?

BODIPY FL dye is a green fluorescent dye with unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds.

Is bodipy polar or nonpolar?

Invitrogen™ BODIPY™ dyes are unusual in that they are relatively nonpolar and the chromophore is electrically neutral (Figure 1.4. 2). These properties tend to minimize dye-induced perturbation of conjugate functional properties.

What does bodipy bind?

Boron dipyrromethene (BODIPY) is a highly lipophilic neutral fluorophore that emits green fluorescence (510–540 nm) when excited by blue light (460–490 nm) after binding to lipids [24].

What are bodipy dyes?

Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.

Is bodipy aromatic?

3. BODIPYs fused to aromatic heterocycles. So far, instead of aromatic hydrocarbons, many aromatic ring-fused BODIPY dyes have been developed by introducing 5-membered aromatic heterocycles, such as furan, thiophene and pyrrole derivatives, at the b bond, as summarized in Fig.

Is bodipy polar?

Invitrogen™ BODIPY™ dyes are unusual in that they are relatively nonpolar and the chromophore is electrically neutral (Figure 1.4. 2).

Is bodipy cell permeable?

These reports indicate that BODIPY dyes without any hydro- philic functionality exhibit membrane permeability, but cannot avoid localization into the intracellular membrane.

Is Tamra cell permeable?

5-TAMRA Azide is a cell-permeable tetramethylrhodamine azide.

Is Cy5 cell permeable?

Cy5, BODIPY650/665, and SiR650 are highly cell permeable, and thus we anticipated that activated fluorescence probes based on them would become cell permeable after MMP enzymatic reaction.