Is Cy3 red or green?

Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

Is Cy3 red or green?

Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

What color is Cy3?

orange
Cy3 is orange in color. It fluoresces greenish yellow at 555 nm excitation and 570 nm emission. Cy3 belongs to the Cyanine family of synthetic polymethine dyes and is widely used for flow cytometry, imaging, and genomic applications.

What is the wavelength for Cy5?

Spectrum [Cy5 (Cyanine-5)] Cy5 (Cyanine-5) is a fluorescent compound with an excitation peak at 651 nm and an emission peak at 670 nm. It can be excited using a 640 nm laser paired with a 670/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAria™ Fusion.

What is Cy5 channel?

Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

Is Cy5 far-red?

The far-red dyes Cy5 and Alexa 647 are popular choices for diverse fluorescence applications because they have large extinction coefficients, high quantum yields, and excitation maxima at frequencies with low cellular absorption.

What Cy3 and Cy5 denotes in microarray technology?

The microarray is analyzed in a scanner, which uses laser light to cause each spot to fluoresce and measures the relative intensity of emission, typically at green (Cy3) and red (Cy5) wavelengths.

What is the size of Cy3?

Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).

Is Cy5 membrane permeable?

Cy5, BODIPY650/665, and SiR650 are highly cell permeable, and thus we anticipated that activated fluorescence probes based on them would become cell permeable after MMP enzymatic reaction.

What is a Cy5 label?

Cy5 is an ideal fluorescent dye for labeling of proteins with its intense signal in the red region of the spectrum.

What is Cy5 used for?

Can you use Cy3 and Cy5 together?

Cy3 dyes are yellow-orange. Specific filter wavelengths (550-600 nm and 655-695 nm) have to be used to avoid background contamination when using Cy3 and Cy5 together to optically separate the two fluorescent cyanines. Cy3 is used interchangeably with PE, though PE remains a better choice.

What is Cy3 Cy5 Cy7 and cy2?

Available non-sulfonated dyes incude Cy3, Cy3.5, Cy5, Cy5.5, Cy7, and Cy7.5. Cy ® stands for ‘cyanine’, and the first digit identifies the number of carbon atoms between the indolenine groups. Cy2 which is an oxazole derivative rather than indolenin, is an exception from this rule. The suffix .5 is added for benzo-fused cyanines.

What is Cy5 dye?

Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 dye or Invitrogen Alexa Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

What is Cy3 (cyanine-3) spectrally similar to?

Cy3 (Cyanine-3) is spectrally similar to iFluor 555, Cellpaint Orange, Alexa Fluor 555, Helix Fluor 575, and iFluor 560. Cy3 (Cyanine-3) belongs to the following related categories: Peptide and Oligonucleotide Labeling, Bioconjugation, and Dyes by Functional Group. Click here to add a spectrum Loading workspace…

What is the difference between Alexa Fluor® and cy2 and Cy5?

This is in contrast to Cy2 and Cy5, which are outperformed in aqueous media by Alexa Fluor® conjugates. Figure 1. Sections of gastric mucosa were stained and mounted in DPX .