Stain of reticulin is a staining that stains the reticulinic fibers of the stroma and makes it possible to specify the architecture of the tumors (as for example the follicular architecture). It is very useful in cases where the tumor is necrotic.
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What are the uses of reticulin stain?
Stain of reticulin is a staining that stains the reticulinic fibers of the stroma and makes it possible to specify the architecture of the tumors (as for example the follicular architecture). It is very useful in cases where the tumor is necrotic.
What is the principle of reticulin stain?
Reticulin Stain Kit is a metal impregnation technique where ammoniacal silver initially binds to the tissue component of interest. Then the reducing agent (formalin) produces a dark insoluble precipitate, enabling silver to be reduced into a visible state.
What is reticular stain?
In pathology, the reticulin stain, is a popular staining method in histology. It is used to visualize reticular fiber and used extensively in liver histopathology.
Which stain is used for amyloid?
Congo Red
Congo Red and Thioflavin S are the two major histological stains used to detect any form of amyloid. These dyes bind to the characteristic β-pleated sheet conformation of amyloid.
What is Van Gieson stain used for?
Van Gieson’s trichrome is used for the differentiation between collagen and smooth muscle in tumors and to demonstrate the increase in the amount of collagen in these pathologies.
What stains staining techniques are used to demonstrate the reticulin fibers?
Movat Pentachrome is a super connective tissue stain. It is used to study heart tissue, blood vessels and vascular and lung diseases. Due to the excellent differentiation of collagen, elastic, fibrin and muscle fibers, the Movat can reveal subtle changes that the routine and normal special stains do not.
What are the special stains?
“Special stains” are processes that generally employ a dye or chemical that has an affinity for the particular tissue component that is to be demonstrated. They allow the presence/or absence of certain cell types, structures and/or microorganisms to be viewed microscopically.
What does warthin Starry stain?
Warthin – Starry stain is a stain based on silver nitrate used in histology. This type of stain is commonly called silver staining. Warthin – Starry stain is used for the visualization of spirochetes but also for the detection of Helicobacter pylori and microsperediates.
What is Congo red stain used for?
In histology and microscopy, Congo red is used for staining in amyloidosis, and for the cell walls of plants and fungi, and for the outer membrane of Gram-negative bacteria. Apple-green birefringence of Congo red stained preparations under polarized light is indicative of the presence of amyloid fibrils.
Does amyloid stain with PAS?
Amyloid deposits are pale when H&E stain is applied and do not stain with PAS or with methenamine silver stain. They are usually more amorphous than those of diabetic nephropathy, which are positive on PAS and silver stain.