HPLC Instrument Considerations Standard columns for reversed phase, normal phase, and ion exchange chromatography typically range from 3.9 to 4.6 mm internal diameter and 15, 25, and 30 cm in length. Standard columns for gel permeation chromatography (GPC) are 7.8 mm by 30 cm.
Table of Contents
What are typical HPLC column dimensions?
HPLC Instrument Considerations Standard columns for reversed phase, normal phase, and ion exchange chromatography typically range from 3.9 to 4.6 mm internal diameter and 15, 25, and 30 cm in length. Standard columns for gel permeation chromatography (GPC) are 7.8 mm by 30 cm.
What is the particle size of C18 column?
HyPURITY C18 HPLC Columns, 3µm Particle Size
| Diameter (Metric) | 4.6 mm |
|---|---|
| Pore Size | 190 Å |
| Surface Area | 200 m²/g |
| USP Type | L1 |
| Stationary Phase | C18 |
What are standard columns in HPLC?
Size exclusion HPLC columns are used primarily for the separation of proteins and carbohydrates. Other types of HPLC columns include affinity, ion exclusion, and displacement chromatography columns; a chiral HPLC column can be used to resolve racemic mixtures.
What is the length of separation column of HPLC 7?
Due to the small sample amount separated in analytical HPLC, typical column dimensions are 2.1–4.6 mm diameter, and 30–250 mm length.
How do I calculate column size in HPLC?
PROCEDURE
- Use Volume = pi x radius2 x Length.
- pi = 3.14.
- r2 and Length should be converted to centimeters.
- Diameter of column divided by 2 = radius.
- radius x radius = r2
- 3.14 x r2 x L = Volume in cm3
- cm3 = 1 mL.
What is the structure of C18 column?
YMC-Triart C18 is a multi-layer hybrid particle consisting of two types of layers: a silica based layer (inorganic) and a hybrid polymer based layer (organic). This layered structure contributes to Triart’s combined physical durability and chromatographic performance.
What makes up a C 18 column?
For example, a C18 column contains beads that have 18-carbon chains attached to them. These stationary phases are powders, like silica, and they can be loaded into a column just like silica can. A C18 column is an example of a “reverse phase” column.
How many columns is 1200 square feet?
You’ll need at least 16 columns in 1200 square feet to ensure the house’s stability. Each row has three columns. 3X4=12 columns are required for a standard regular design.