The CombiFlash EZ Prep offers streamlined Flash and Prep HPLC chromatography in one compact, easy-to-operate unit. With just two clicks, users can switch from pre-purifying samples on Flash to generating submission-quality compounds on Prep HPLC.
Table of Contents
What is CombiFlash chromatography?
The CombiFlash EZ Prep offers streamlined Flash and Prep HPLC chromatography in one compact, easy-to-operate unit. With just two clicks, users can switch from pre-purifying samples on Flash to generating submission-quality compounds on Prep HPLC.
What is RediSep?
RediSep columns are precision-packed for high resolution and reproducibility. They feature a one-piece design with no cumbersome or expensive adapters, valves, barrels, or other accessories.
What is flash chromatography used for?
Flash chromatography is a chemical separation technique used to purify chemical mixtures. Because it is a purification technology, the process is also referred to as flash purification.
What is different about flash chromatography?
Flash chromatography is a purification technique that is designed for rapid separation by using air pressure as opposed to slow and inefficient gravity-fed chromatography. It differs from the conventional column technique by using slightly smaller silica gel particles and pressurized gas at 50–200 psi.
How does a flash column work?
Flash chromatography uses a glass column that is packed with silica as the stationary phase and a solvent – either polar, non-polar or a mix of the two depending on the compounds to separate. This is the mobile phase and is initially added to cover the silica.
Why is it called flash column chromatography?
If the solvent is allowed to flow down the column by gravity, or percolation, it is called Gravity column chromatography. If the solvent is forced down the column by positive air pressure, it is called Flash chromatography.
How do you set up Flash in column chromatography?
How to Run a Flash Column
- Choosing a Chromatography Solvent System. Identify a suitable solvent mixture for your compound or reaction mixture.
- Packing the Column.
- Loading the Column.
- Running the Column.
- After the column-cleaning up.
Which gas is used in flash chromatography?
The software also saves the resulting chromatograph from the process for archival and/or later recall purposes. PRINCIPLE OF FLASH CHROMATOGRAPHY: The principle is that the eluent which is a liquid, under gas pressure (normally nitrogen or compressed air) rapidly pushed through a short glass column.
How do you use column flash?
Why nitrogen is used in flash chromatography?
The most prominent advantage of using nitrogen as a carrier gas is that of all the carrier gases it is the most efficient when used at its optimum linear velocity (12 cm/s), meaning it will produce the narrowest chromatographic peaks.