What is fluoro-Jade C?
Abstract. Fluoro-Jade C, a new-developed fluorescent dye, has been successfully applied for identification of neuronal degeneration in the substantia nigra of 1-methy-4-phenyl-1,2,3,6-tetrahydro pyrindine (MPTP)-treated mice in the present study.
How does fluoro-jade work?
Fluoro-Jade is an anionic fluorochrome capable of selectively staining degenerating neurons in brain slices. The histochemical application of Fluoro-Jade results in a simple, sensitive and reliable method for staining degenerating neurons and their processes.
What does FJB stain?
Fluoro-Jade B (FJB) is an anionic fluorescein derivative that has been reported to specifically stain degenerating neurons.
What is Fluoro-Jade B?
Abstract. Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. However, Fluoro-Jade B has an even greater specific affinity for degenerating neurons.
What does Fluoro-Jade B stain?
Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade B exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons.
What is a nissl stain?
Staining. “Nissl stains” refers to various basic dyes that selectively label negatively charged molecules such as DNA and RNA. Because ribosomes are rich in ribosomal RNA, they are strongly basophilic (“base-loving”).
What does NeuN antibody stain?
NeuN (neuronal nuclei) antibodies are fundamental tools for staining mature neurons and studying neuronal development and differentiation.
What is NeuN marker?
NeuN antibodies are used to identify mature neurons in cell cultures and tissue sections in applications ranging from immunofluorescence to flow cytometry. They can be used in combination with other cell type markers to study neural networks and neuronal differentiation and development.