What is SPE and SPME?

Solid phase extraction (SPE) and solid phase microextraction (SPME) are among the most significant sample clean-up and pretreatment techniques, which have attracted a considerable interest in the pre-concentration and determination of a wide variety of analytes in biological, pharmaceutical, environmental, industrial …

Table of Contents

What is a C18 SPE column?

SPE Supra-Clean C18-S Column, 2 g/15 mL Spherical Silica-based Solid Phase Extraction (SPE) Columns are recommended for extracting mid-polar to non-polar compounds when analyzing drugs and drug metabolites in biological matrices, trace organic material in water, and toxins in food.

What is SPE method?

Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. HPLC, GC, TLC). In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.

What are the types of SPE?

The types of SPE fall into several categories including, reversed phase, normal phase, ion exchange (anion/cation), as well as mixed-mode phases, which have the properties of more than one type of SPE material. Figure 4 describes the properties of these phases.

What is the main difference between SPME and μ SPE?

SPE require 500 mL of sample and leads to a limit of detection in the range 0.005 – 0.02 ng/mL. SPME requires only 4.0 mL of sample and provides a limit of detection in the range 0.004 – 0.03 ng/mL. No significant difference between pesticides found in spiked samples using SPE and SPME were obtained.

How is SPME?

With SPME, the analytes are absorbed from the liquid or gaseous sample on to an absorbent coated fused silica fibre, which is part of the syringe needle, for a fixed time. The fibre is then inserted directly into a GC injection port for thermal desorption.

What is SPE column?

SPE cartridges, also referred to as SPE columns, are single-use plastic tubes. Thermo Scientific SPE cartridges are available in a variety of sizes with a capacity of from 1mL to 150mL. Cartridge format is used to process a limited number of samples at a time.

How do you use a SPE cartridge?

To perform normal-phase chromatography with SPE cartridges, use a step gradient of nonpolar solvents with a polar packing material.

Condition the cartridge with six to ten hold-up volumes of non-polar solvent, usually the same solvent in which the sample is dissolved.
Load the sample solution onto the cartridge bed.

How do I choose a SPE?

The general method of SPE is: use the principle of chromatographic analysis of selective adsorption and selective elution, pass the liquid sample through the adsorbent, retain the analytes in it, wash the impurities with a suitable solvent, and then elute the analytes with a small amount of solvent, to achieve the …

How do you use SPE?

How do I choose a SPE cartridge?

SPE cartridge format

Cartridge volume should be selected to closely match the volume of wash solvent use.
The volume of sample and solvent added can help in determine which size cartridge you need.
Typically, one can load a sample that is approximately 5-10% of the sorbent weight in a given SPE cartridge.