eIF4E, the cap-binding protein promotes protein synthesis by binding to eIF4G. Beside its role in translation regulation, eIF4E is also important for mRNA stability and the proper subcellular localization of mRNA.
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What is the purpose of cap-binding protein?
eIF4E, the cap-binding protein promotes protein synthesis by binding to eIF4G. Beside its role in translation regulation, eIF4E is also important for mRNA stability and the proper subcellular localization of mRNA.
What regulates binding of the ribosome to mRNA?
Translation of most Escherichia coli ribosomal protein genes is regulated by autoregulatory feedback mechanisms in which one gene in the operon encodes a ribosomal protein (r-protein) that can bind either to rRNA during ribosome biogenesis or to its mRNA and repress translation (31, 77).
What are RNA binding proteins called?
RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes. RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others.
How do DNA binding proteins bind to DNA?
Among the proteins that bind to DNA are transcription factors that activate or repress gene expression by binding to DNA motifs and histones that form part of the structure of DNA and bind to it less specifically. Also proteins that repair DNA such as uracil-DNA glycosylase interact closely with it.
What is the function of a CAP in transcription?
The cap structure protects mRNAs from degradation by exonucleases and promotes transcription, polyadenylation, splicing, and nuclear export of mRNA and U-rich, capped snRNAs.
What is a CAP-binding site?
In these operons, a CAP-binding site is located upstream of the RNA-polymerase-binding site in the promoter. This increases the binding ability of RNA polymerase to the promoter region and the transcription of the genes.
Where do RNA-binding proteins bind?
Where do regulatory proteins bind?
The repressor or activator protein binds near its regulatory target: the gene. Some regulatory proteins must have a ligand attached to them to be able to bind, whereas others are unable to bind when attached to a ligand.
What is capping in protein synthesis?
Capping is the first modification made to RNA polymerase II-transcribed RNA and takes place co-transcriptionally in the nucleus as soon as the first 25–30 nts are incorporated into the nascent transcript (6,7).
What is the function of the 5 cap and poly A tail?
5′ cap and poly-A tail Both the cap and the tail protect the transcript and help it get exported from the nucleus and translated on the ribosomes (protein-making “machines”) found in the cytosol 1start superscript, 1, end superscript. The 5′ cap is added to the first nucleotide in the transcript during transcription.